Sıçan FLT3 geninin klonlanması ve sıçanlarda karaciğer reejenerasyonunda FLT3 geninin ifade profilinin incelenmesi

Liver has a unique capacity to regenerate itself upon exposure to viral infections,toxic reactions and cancer formation which result in the loss of hepatocyte. Liverregeneration is a complex phenomenon in which several factors participate duringits onset. Cellular proliferation is one of the important component of this processand the factors regulate this proliferation has a vital role. FLT3, a well-knownhematopoietic stem cell and hepatic lineage surface marker, takes place in pro-liferative events of hematopoietic stem cells. However, its contribution to liverregeneration is not known. Therefore, in this study, we aimed to examine the roleof FLT3 during liver regeneration. First we cloned a partial cDNA of rat homologof FLT3 from thymus and examined the tissue specific expressions both in mRNAand protein level. After performing hepatectomy in rats to induce liver regenera-tion, expression profile of FLT3 in both mRNA and protein level and its cellularlocalization were also investigated during the different stages of liver regenerationmodels. Our data showed that FLT3 is expressed in most of the rat tissues andin liver regeneration. However, its intracellular localization is changed during thelate stages of liver regeneration. Therefore, our results suggest a mechanism inwhich FLT3 receptor is activated in the late stages of liver regeneration.Keywords: liver regeneration, FLT3, oval cells, rat.iii