Effects of atp-dependent potassium channel (KATP) agonist (pinacidil) and antogonists (glibenclamide) of penisilin g-induced epilepsy model in rats

In general epileptic seizures occurs as a result of the impair of balance between the excitatory and inhibitory systems, and it turns over to the increased activity of excitatory systems in brain. The developments of experimental epilepsy models are considered important in terms of contribute to the physiopathology and epilepsy treatment. Penicilin is the one of the substances which causes the epilepsy. 32 pieceof the range of 200-250 g weightiness Wistar albino rats were used in the study. Experimental animals were randomly separated to four groups. The groups were formed 500 I.U PenicilinG+salin, 500 I.U Penicilin G+DMSO, 500 I.U Penicilin G+0.01 mg/kg pinacidil, 500 I.U Penicilin G +1.0 mg/kg glibenclamide were applied, respectively. All of the rats were applied 1.2 g/kg intraperitoneally urethane to anesthesia. After bone of skull was removed, the dura mater was removed carefully. ECoG recordings were taken using clamp electrode. Statistical analysis, each group in itself with paired t-test, the differences between the four groups was performed using one-way analysis of variance. Significant differences were evaluated by Tukey's post hoc test. Results of studies, in Penicilin-G induced experimental model of epilepsy, giving intracortical 0.01 µl (microliters) pinacidil formed agonist effect on neuronal KATP channels (p<0.05), in Penicilin-G induced experimental model of epilepsy, the result of appling intraperitoneal 1.0 mg/kg glibenclamide was not significant effect on epileptic discharges. Consequently, a KATP channel opener pinacidilbrings back the ability to impaired buffering of extracellular K+ions in focal of epileptogenik cells, and obstacles to the increase in extracellular K+ ions, brings to normal levelsof lower treshold of neuronal excitability thatleads to the epilepsy seizures. Although, glibenclamide is a selective and strong bloker on KATP channels and has a lot of antogonisteffects, thereis no effect on inhibition of the epilepsy seizures. Key Words: Epilepsy, Glibenclamide, KATP Channel, Penicilin G, Pinacidil