The investigation of the effect of fraxin in the liver damage induced by cisplatin in rats

This study was designed to assess the efficacy of Fraxin have various biological properties in cisplatin-induced liver injury. In our study, 24 Wistar albino rats were randomly divided into Control, Fraxin, Cisplatin and Fraxin + Cisplatin groups. Cisplatin 12 mg/kg i.p, Fraxin 40 mg/kg orally was administered. At the end of the experiment, the rats were sacrificed and the liver tissues taken rapidly. Until biochemical, histopathologic and immunohistochemical evaluations were made. Liver tissue specimens were maintained under appropriate conditions. According to our biochemical findings, MDA level increased and antioxidant enzyme activities decreased in Cisplatin group, antioxidant enzyme activities increased, MDA level decreased in Fraxin + Cisplatin group. Immunohistochemical evaluations showed that, TNF-α and Caspase-3 expression were negative in the Control and Fraxin groups, whereas severe levels were observed in the Cisplatin group. However, it was seen that TNF-α and Caspase-3 expression were mild levels in the Fraxin + Cisplatin treatment group. In addition, it was observed that the increase of pathological markers such as hydropic degeneration, coagulation necrosis, dilatation in sinuzoid and hyperemia in the cisplatin group were compatible with our biochemical and immunohistochemical findings. Biochemical, immunohistochemical and histopathological results revealed that Fraxin was effective in relieving Cisplatin-induced liver damage.