Investigation possible effects of traumatic brain injury on testicles in rats

Purpose:We aimed to show the effects of traumatic brain injury on the testis.In this study, we aimed to investigate the impact of travmatic brain injury which was formed by weight drop model on testis.Material and Method:Animal groups were designed as showed belowGrup I, Control group: No application was performed to this animals (n=7).Grup II, Trauma group: Traumatic brain injury which is done by weight drop model (n=7).24 hours later trauma, animals were sacrified. Testis tissue slides were stained with Hematoxylene&Eosine (H&E), Masson Trichrome and Periodic Acid Schiff (PAS) for light microscopy. Aktif Kaspaz 3 and Immunohistochemical TUNEL stain (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling) were performed to determine apoptosis.And GnRH, testosterone, LH, FSH hormone levels and MDA, GPx levels were observed and statistically groups were compared.In histological evaluation, control group?s testis tissue was observed normal.Testis had an connective tissue capsule which is called tunica albuginea.On the other hand, in traumatic group there was a diminution in serial spermatogenic cells when histologically evaluated.In Tunel analysis, we evaluated DNA fragmentation and there was apoptosis in all the groups including control group. In group two we observed higher TUNEL positive cells when compared to group one cells.Result:In our study, we observed the impact of traumatic brain injury which is turned out by weight drop model on testis using immunohistochemistry, ultrastructural and biochemistry techniques. These results suggest that, traumatic brain injury causes damage in the tubulles and increase in ratio of apoptosis.In our opinion, more studies are needed to understand the mechanism of traumatic brain injury on testis damage.