Different subtypes of HPV in cervical dysplasia of E6 / E7 Gene Expression Analysis

Objective: The objective of our study is to determine E6 and E7 carcinogenic proteins caused by different sub-types of HPV as well as investigating their effects on cervical dysplacias. Material-Method: 77 patients aged between 21-65, whose Smear results were reported as ASCUS, ASC-H, LSIL, HSIL and AGC, were incorporated into the study. The identification and typology of HPV DNA were performed by using HPV sign® Q24 complete kit and RotorGene and PyroMark Q24 (Qiagen, Germany) systems. HPV E6/E7 mRNA gene expression within the samples prepared from the cervical biopsies of HPV DNA positive cases was investigated through NASBA method (Nucleic acid sequence based amplification) by using NucliSENS® EasyQ Genetic Analyzer device (Biomerieux, France). Discussion: As the result of the cervical biopsies of HPV DNA positive cases, an abnormal cervical lesion (CIN 1,2,3) was detected in 45,5% (10/22) of these cases, whereas a CIN 3- cervical lesion was seen in 13,63% of them. As the result of the cervical biopsy results of HPV mRNA positive cases, an abnormal cervical lesion (CIN 1,2,3) was detected in 45,5 % (5/11) of these cases, while in 27% of them was CIN 3 seen. When the cervical biopsy results of the groups, the HPV DNA and HPV mRNA markers of which were both positive, were compared with those having CIN 3, no statistically significant difference could be found (p=0,375). However, the high rate of CIN 3 within the group that is HPV mRNA-positive attracts attention. Result: The tests used for determining HPV E6/E7 mRNA in the cervical lesion show a better correlation with the severity of cervical dysplasia. These tests have a significant potential in foreseeing cervical cancer. Yet, studies on a larger scale are required to be performed on this subject. Key Words: HPV, HPV DNA, E6/E7 mRNA, Cervical Dysplasia.