Obtaining haploid plant via anther and ovule culture techniques in cyclamen

In this study, haploidization possibilities via ovule and anther culture were investigated in C. persicum, C.coum, C. hederifolium, C. pseudibericum, C. cilicium and Melody F1. Anthers were cultured on B5 medium including 0, 1, 3, 5 µM NAA, charcoal (0.5, 1 mg/l), silver nitrate (AgNO3) after 2 days +4°C cold pretreatment. Callus were observed from the anthers of Melody F1 commercial variety cultured on B5 medium including 1 g/l charcoal, 0.5 mg/l charcoal + %0.1 AgNO3 +1 µM NAA, 0.5 mg/l charcoal + %0.1 AgNO3+ 3 µM NAA and 0.5 g/l activated coal + %0.1 AgNO3 + 5 µM NAA. In C. persicum species, embryo and plants derieved from these embryos formed from anther explants were cultured on BS medium containing 5µM NAA. Shoot like formations were formed from anthers cultured on B5 medium including 5µM NAA in C. hederifolium species. As a result of ovule culture experiments of C. persicum and Melody F1 commercial variety, callus, embryo formations and shoots occured from MS medium supplied with 2 mg/l 2,4-D + 0.8 mg/l 2iP and 2 mg/l 2,4-D + 0.5 mg/l 2iP, respectively. In C. coum and C. pseudibericum species, only callus formations were formed. Embryos occured from MS medium containing 0.5 mg/l 2,4-D + 0.5 mg/l 2iP, 0.5 mg/l 2,4-D + 0.8 mg/l 2iP, 1 mg/l 2,4-D + 0.3 mg/l 2iP in C. cilicium. Callus, embryos, shoot like formations formed in MS medium containing 1 mg/l 2,4-D + 0.5 mg/l 2iP in C. hederifolium species.