Development real-time analysis method of frequently used antiepilectic drugs with LC-MS/MS

In this study, it has been developed simple, rapid, quantitative, and reliable method for active substances of frequently used antiepilectic drugs detection such as carbamazepine, oxcarbazepine, phenytoin, diazepam, lorazepam, clonazepam, levetiracetam, gabapentin and lamotrigine in human blood by using liquid chromatography-mass spectrometry (LC-MS/MS) device. It has been validated in terms of recovery, accuracy, percentage of relative standart deviation, limit of quantification and limit of detection for each active substance of drugs. LOD and LOQ values for obtained concentrations of each active substances were resulted that 1 ng.mL-1; 0,05 ng.mL-1-0,16 ng.mL-1, 25 ng.mL-1; 0,12 ng.mL-1-0,46 ng.mL-1, 75 ng.mL-1; 0,20 ng.mL-1-0,68 ng.mL-1 and 1 ng.mL-1; 0,17 ng.mL-1-0,52 ng.mL-1, 25 ng.mL-1; 0,40 ng.mL-1-1,54 ng.mL-1, 75 ng.mL-1 ; 0,67 ng.mL-1-2,26 ng.mL-1 respectively. This method was resulted in %98 recovery at 1 ng.mL-1 concentration and %99 recovery at higher concentration levels and also confirmed to EURACHEM guide.