Determination of antioxidant activity of Stachys lavandulifolia Vahl. var. brachydon Boiss. by different in vitro methods

Determinations of in vivo and in vitro antioxidant, antiradical, and antimicrobial activities of natural plant sources is one of the common area for biochemists. Antioxidant compounds are abundantly present in the fruits, seeds, leaves, and flowers of natural plants. Many of the previous studies have been shown that the consumption of plant sources that contain antioxidants reduce the risk of tissue damage, cell death, aging, cancer, cardiovascular diseases, neural disorders, skin irritations and inflammations. The main purpose of this study is determining antioxidant activities of water and ethanol extracts that obtained from the leaves of Stachys lavandulifolia Vahl. var. brachydon Boiss. species grown in Turkey. Antioxidant activities of extracts were determined according to the ABTS, DPPH, FRAP, CUPRAC and FTC in vitro methods and the results were compared with BHA, BHT and ascorbic acide which are used as standard antioxidants. Also, phenolic composition of Stachys lavandulifolia Vahl. var. brachydon Boiss. determined by UHPLC-MS methods. According to the result, many polyphenolic compounds such as chlorogenic acid, quinic acid, hyperoside and protocatechuic acid were mainly detected. The antioxidant capacity of Stachys lavandulifolia Vahl. var. brachydon Boiss. is associated with the presence of phenolic compounds, which react with the free radicals formed during the oxidation process Keywords: Antioxidants, free radicals, lavandulifolia, phenolic compounds, Stachys