Invenstigation of mather's breastmilk of term and pretem infants with respect to some biochemical parameters

Invenstigation of Mather's Breastmilk of Term and Pretem Infants With Respect to Some Biochemical Parameters Aim: Due to it's optimal protein and amino acid composition, human milk is considered to be the best source of nutrition for the human infant. Although the nutritional value of human blood has been extensively researched, very few publications have been published describing the free amino acid (FAA) content. The aim of the study is to determine the concent of some free amino acids, vitamin B12 and folate in maternal milk. Materials and Method: Human milk was obtained during two different stages of lactation (colostral and transitional milk). One hundred samples were collected from 50 healthy mothers of term infants and 50 mothers of premature infants 29 to 37 weeks gestation (mean 33 weeks). Free amino acid levels were measured by using LC-MS / MS with respective analyze kits. Vitamin B12 and folate levels were determined by chemiluminescence immunoassay (CLIA). Result: Lactation period and gestational age affected free amino acid levels (especially; arginine, argininosucsinic acid, asparagine, glutamic acid, hydroxyproline, serine, carnitine, dopamine, histidine, leucine, lysine, phenylalanine). It is determined that, glutamic asid is the most abundant amino acid (term mothers; 2313 mmol/L, pretem mothers 1270 mmol/L), followed by alanine (term mothers; 239 mmol/L, pretem mothers 305 mmol/L). In comparing individual free amino acid, vitamin B12, folate, it was found that there were significant differences in concentrations between term human milk and preterm (p<0.001). Conclusion: As a result, it is estimated that human milk composition is significantly affected by gestational age and lactation period. It has been reported that the free amino acid content of breast milks how wide variations. Due tok this, new researches are needed in this regard. Key Word: Breastmilk, free amino acid, gestational age, lactation.