Testis kanseri hücre dizilerinde n-asetilsisteinin bleomisinin sitotoksisitesi üzerine etkileri

In cancer cells, oxidative stress, stimulation of cell proliferation, mutations and genetic instability increase,such as cellular sensitivity change to anticancer drugs may be significant consequences. In contrast, increase oxidative stress, it can also provide an opportunity to kill cancer cells. Used in our study bleomycin generates reactive oxygen species such as superoxide and hydrogen peroxide. Bleomycin is a drug commonly used to treat of testicular cancer and It?s shown to lead to the formation of ROS high levels of this drug. A result of ROS formed building blocks to ensure the survival of the cell are damaged. Membranes composed of lipids and proteins sensitive to damage ROS. In addition, the height of the levels of ROS are known to induce cell apoptosis. Antineoplastic agents inducing oxidants cancer cells killed by apoptosis many studies have reported.In our study, we thought during therapy the use of bleomycin with antioxidants can adversely affect the process of treated. N-acetylcysteine (NAC) as an antioxidant widely used in vivo and in vitro. NAC produced by living organisms and the natural sulfur-containing amino acid derivative is a compound known to have powerful antioxidant properties. Therefore, on cytotoxic effects in testicular cancer cell culture formed by Bleomycin, which is controversial as an antioxidant effect of NAC treatment were investigated. Like NAC play a role in detoxification protects a cell and the components of the cell against oxidative stress. Due to the property, in our study examined the effects of NAC on oxidative stress created by Bleomycin. The effects of oxidative stress was measured by protein carbonyl kit.In our study, we identified the cytotoxic dose of bleomycin in testicular cancer cell lines. Control, bleomycin, NAC and bleomycin with NAC were created four experimental groups. This experimental groups, 24, 48 and 72-hour incubations with the cytotoxicity was determined by MTT test. Oxidants, cells killed by apoptosis, which suggests some of the studies are not quantitative, percentage of dead cells of apoptosis and necrosis have not been determined in many studies. In our study we made using the Caspase-3 and Annexin V kits in terms of determining the percentage of cells died of apoptosis and necrosis has been working a fully quantitative. After 6 hours of incubation in all experimental groups, indicating early apoptosis Annexin V test was performed using Flow Cytometer. Bleomycin group has the highest percentage of apoptosis and apoptosis were statistically significantly decreased in the NAC + bleomycin group. After 24-hour incubation Caspase-3 experiment results support Annexin V also testing.We think it is important for prognosis of the disease of the chemotherapeutic treatment of patients with testicular cancer all the experiment results. During treatment Bleomycin kills testicular cancer cells by generated ROS. Because of antioxidants prevent ROS, we believe that the use of antioxidants during treatment with Bleomycin negatively affect the treatment process.