Histologic and biochemical research of trifluraline effects on rat duedonum and liver

In our study, we examined toxicologic effects of Trifluralin, a dinitroanniline derive which has a wide usage area in agriculture worldwide, on duedonum and liver tissues histologically and biochemically.Study started with the decision of Dokuz Eylül Medical Sciences Institude Ethics Commission dated on 21.11.2008, no. 128.In our investigation, approximately 200-250 grams were used 30 Wistar strain male rats. Rats in each group to be 7 animals were divided into 4 groups. Anything applied to the first group, the control group. The second group given corn oil Sham group. The third group, 2 g / kg / day given TRF + corn oil, High-Dose (HD) group. The fourth group, 0.8 g / kg / day given TRF + corn oil, low-dose (LD) group. Applied substances for a period of 21 days was given by intragastrik gavaj. Rats were sacrified after the application. Fragments of collected tissues from duedonum and liver were embedded in paraffin blocks and sectioned after routine histologic technics for counting TUNEL positive cells and stained for histologic and morfometric examining with H&E. Other parts of tissues were used for MDA, GPx and SOD examination. Blood samples were collected at the end of the experiment for examining AST, ALT, ALP and Albumin levels. And body weights of rats were recorded before and after the experimental process.According to histologic and morfometric assesment; HD and LD group liver and duedonum tissues were found damaged than sham and control groups. In HD and LD groups TUNEL positive cell numbers were comperatively more than sham and control groups. According to MDA, SOD and GPx assesment; HD and LD groups? MDA rates were found higher and GPx levels were found lower than sham and control groups in liver tissue. SOD levels were found significantly lower in only LD group. In duedonum tissue; MDA and SOD levels showed differences significantly only in LD grup. GPx level assesment didn?t give significant statistical datas. According to our study results; we can say that TRF induces oxidative stress, causes histological damages and biochemical changes in liver and duedonum tissues.Key words: Trifluraline, Liver, Duodenum, Apoptosis, Oksidative stress