Türkiye?nin Batı Karadeniz bölgesinde, kefal balığı karaciğer mikrozomunda, cyp1a seviyesi ve 7-etoksirezorufin O-deetilaz (erod) aktivitesi ile toksik organik kirleticilerin izlenmesi

The Black Sea has been increasingly threatened by chemical pollutants over the past decades as a result of industrial, anthropogenic, and agricultural activities. Industrial areas, rivers and coastal cities have tendency to create local pollution in the Black Sea. In this study, the extent of PAHs, PCBs and dioxins type pollution was determined and monitored in Zonguldak Harbour, Ereğli Harbour, Sakarya River Mouth, Melen and Gülüç Stream Mouths and Amasra by measuring CYP1A associated 7-ethoxyresorufin O-deethylase (EROD) activity and immunochemical detection of CYP1A protein level in mullet liver microsomes in August 2005, 2006 and 2007. The lowest EROD activities were measured in mullet samples caught from Amasra. This region was considered as reference site. Mullets collected fromZonguldak city harbour, a highly urbanized and industrial city in the West Black Sea Region of Turkey, had about 6-9 times higher EROD activities than those obtained from reference site, Amasra. The highly elevated EROD activities were also detected in mullets captured from Ereğli Harbour which were about 7-9 times higher with respect to the value obtained from Amasra. Gülüç Stream was the other highly polluted station in this study. EROD activities of mullet samples caught from this station were about 6 times higher than Amasra. EROD activities of mullets caught from Melen Stream Mouth were about 2-2.5 times higher than those obtained from Amasra. EROD activities in mullets sampled from Sakarya River Mouth were 2-4 times higher than those caught from Amasra. CYP1A protein levels were also determined in mullet liver microsomes by immunochemical analysis. CYP1A protein level measurements were highly correlated with EROD activity measurements. Besides these biochemical parameters, total PAH concentrations were measured in fish liver tissues. Total PAH concentrations of mullets caught from Zonguldak Harbour, Ereğli Harbour, Sakarya River Mouth, Gülüç and Melen Stream Mouths were higher than those obtained from reference site, Amasra.In this study, the induced CYP1A protein level and associated EROD activities, and high total PAH concentrations in mullet livers showed the presence of PAHs, PCBs and dioxins type organic pollutants in Zonguldak Harbour, Ereğli Harbour, Sakarya River, Melen Stream and Gülüç Stream. These results indicate that these sites have tendency to create local pollution in the West Black Sea Region of Turkey.Keywords: Black Sea, CYP1A, EROD, Mullet, PAH, Pollution.