Türkiye ve Kırgızistan'dan alınan kadın tuzluğu genotiplerinin moleküler ve morfolojik karakterizasyonu

This study was conducted to investigate the genetic diversity and the relationship between 2 populations including 10 genotypes of Berberis crataegina collected from the province of Kayseri in Turkey and 22 genotypes of Berberis sphaerocarpa Kar. et Kit collected from Kyrgyzstan using Inter Simple Sequence Repeat (ISSR) Molecular Markers. The characterization was performed using 20 ISSR primers on 32 Barberry genotypes and obtained 150 bands of which 111 bands were polymorphic.Band size varied between 190 bp and 1400 bp, band numbers for each primer produced varied between 5 to 14 and the average of alleles produced from 20 (ISSR) primers was 7.5.The analysis was performed using NTSYS Pc-2.11X software, and a dendrogram was constructed based on UPGMA technique.3 primers (TAA)8, (CA)6AC, and (CAC)6 did not show any results whereas, 2 primers (AG)7YC and (TCC)5RY were monomorphic. On the other hand, the rest of primers were polymorphic with polymorphism percentages ranged between (28.6%-100%) and an average of 74%.Also, this study included a morphological comparison between fruits of same involved genotypes, this comparison is based on some morphological characteristics (weight, length, and width). All observations made in this study provide that using (ISSR) molecular markers is a reliable method for separating types within a specific genus, and a valuable evidence for decision making in choosing markers for future works, characterization of germplasm, and for contributing of developing Barberry improvement programs. 2017, 56 pages Keywords: Morphological and molecular characterization,Barberry,Berberis sphaerocarpa Kar. et Kit, Berberis crataegina.