In vitro comparison of the accumulation of three different microorganisms to four different kinds of prosthetic esthetic materials

SUMMARY This study was carried out in order to evaluate the adhesion of microorganisms to four different prosthetic esthetic materials. Experimental prosthetic materials are High-fused porcelain (Vita), Medium-fused porcelain (Vita), Polyoxymethylene (Dental-D) and Acrylic resin (Duropont); the microorganisms that used were, C.albicans, S.aureus and S.salivarius. For our study, the experimental models of 10mm radius and 1mm height prosthetic materials were prepared. The prepared samples were sterilised in aluminum foil at 121°C for 15 minutes. The adhesion of microorganisms to sterilised samples are investigated for 24 hours at 37°C in liquid culture dish that fits to characteristics of proliferation of the microorganisms, the number of adherent cells on experimental models were calculated in solid culture dish after snaked in 100 ml saline and diluted. As a result of this study, it was determined that Acrylic resin had undergone more microorganisms adhesion when compared with High-fused porcelain, Medium-fused porcelain and Polyoxymethylene, and Polyoxymethylene has more adhesion than High-fused and Medium-fused porcelains. 62