Evaluation of the cytotoxicity of three different denture base resin materials in vitro.

The purpose of this study was to evaluate the cytotoxic effects of the heat-polymerized and auto-polymerized acrylic resins; and acetal resin as denture base materials on L-929 mouse fibroblast cells over 1st hour, 1st, 3rd, 5th, 7th, 14th days period.Five samples of each resin were fabricated under aseptic conditions in sterile moulds 12 mm in diameter and 1 mm thick for every incubation period. Cytotoxicity of resins was measured by MTT test. Statistical significance was determined by one-way ANOVA. Tukey and Dunnet tests were used to determine differences among the groups. Statistically significant difference was found among test groups at all time incubation periods.The results showed that; the auto-poymerized resin showed more cytotoxic effect than heat-polymerized resin in all time periods. The autopolymerized resin and heat-polymerized resin showed highest cytoyoxic effect on the first day; however acetal resin showed highest cytotoxic effect on the 5th day than heat and auto-polymerized resin; the cytotoxic effect of acetal resin decreased on 7th and 14th days periods.