In vitro evoluation of effects of dental operation microscope and adhesive systems on shear bond strength and microleakage in fissure sealant procedure

Uysal D. 2019. In vitro evaluation of effects of dental operation microscope and adhesive systems on shear bond strength and microleakage in fissure sealant procedure. Yeditepe University, Institute of Health Sciences, Ph.D. Thesis, Istanbul. The aim of this study is to evaluate the shear bond strength and microleakage in fissure sealant procedure using dental operating microscopy and adhesive systems. The third human molar tooth used in the study and four groups of 15 samples and two groups of 15 samples were formed for the microleakage experiment and for the shear bond strength test, respectively. Fissure procedure was performed following the acid etching in two of the experimental groups subject to microleakage test, while fissure sealant was applied by using a dental operating microscopy in one group. In the other two groups, fissure sealant was applied after the application of the bonding and one of these groups was performed by using dental operating microscopy. A stereomicroscope was used for the evaluation of microleakage. Two samples from each group were visualized in a scanning electron microscope (SEM). Fissure sealant was applied to one of the groups formed to test the shear bond strength only after acid etching, while it was applied to the other group after the bonding agent application following the acid etching procedure. Shapiro-Wilk normality test was used for testing the normal distribution one way variance analyse test was used for multiple intergroup comparison, Tukey multiple comparison test was used for subgroup comparisons, Kruskal Wallis Test was used to compare the variables with no normal distribution, Dunn's multiple comparison test was used for subgroup comparisons, and Chi-Square test was used to evaluate the qualitative data. A p value of <0.05 was considered statistically significant. In conclusion, no statistically significant difference was found between the groups for the microleakage test. However, when the groups were evaluated according to the shear bond strength results, the bond strength of the samples subject to fissure sealant procedure after the bonding agent application following the acid etching was found to be significantly higher (p<0.05).