The detection of invasive pulmonary aspergillosis incidence rate with galactomannan and aspergillus PCR methods in icu patients

The aim of the study is to determine the diagnostic value of GM antigene and aspergillus PCR method in BAL and to estimate the incidence rate of IPA with these methods in non-neutropenic ICU patients.This study is conducted prospectively with 44 patients older 18 years-old-age whose BAL has been taken in thoracic ICU of Gazi University Faculty of Medicine.ata is presented as frequency, percentage, mean and median (min, max). Chi-square, Kruskal-Wallis nd Mann-Whitney U tests are used. ROC curve is used to determine the diagnostic values. Sensitivity, specifity, PPV, NPV, +LR, -LR nd DOR are calculated for serum GM, BAL aspergillus PCR and different levels of BAL GM.The incidence rate of IPA is 11,4%. The duration of ICU hospitalization and IMV before BAL, septic shock, total doses of steroid are found to be risk factors of IPA. IPA should be taken into account in patients who are non-responsive to treatment with broad spectrum antibiotics.Detection of BAL GM antigene should be preferred for diagnosis of IPA in non-neutropenic ICU patients. The 0,7 cut-off level for BAL GM has the highest diagnostic value. The diagnostic value of serum GM is low in non-neutropenic ICU patients. Aspergillus PCR has lower diagnostic sensitivity and is not superior to BAL GM antigene in IPA.