Investigation of the effect of loaded nanoparticles on some microorganisms and cell types

In our research; tetrachloro-2-pyridylmethylspiro-N/N-ethylenediamino-cyclotriphosphazenes obtained from the reaction of N-alkyl-N'-2-pyridylmethylethylenediamines with hexachlorocyclotriphosphazenes; fully substituted tetraaminocyclotriphosphazenes obtained by the reaction of pyrrolidine, morpholine and DASD were synthesized. The biological activities of fully substitued tetraaminocyclotriphosphazenes derivatives were evaluated. Antimicrobial activity of synthesized compounds and compounds encapsulated into chitosan/TPP nanoparticles obtained by ionic gelation method on various microorganisms, cytotoxic activity on L929 fibroblast and A549 non-small cell lung cancer cell lines and interaction with plasmid DNA were investigated. The antimicrobial activity of the compounds was investigated by disk diffusion and minimal inhibitory concentration (MIC) methods. The MTT method was used to determine the cytotoxic activity of the compounds in L929 fibroblast and A549 non-small cell lung cancer cell lines. The effects of the compounds on DNA were examined by agarose gel electrophoresis by leaving the pBR322 plasmid DNA to interact with the compounds for a certain period of time; In order to determine which nucleotides the compounds bind to on the DNA, plasmid DNA interacted with the compounds was cut with BamHI and HindIII restriction endonuclease enzymes. Chitosan nanoparticles obtained by ionic gelation method; FT-IR analysis, determination of size and load distribution, taking SEM images and stability studies were carried out. By encapsulating the compounds into nanoparticles, the determination of the efficiency of the active substance and the determination of the release of the active substance were carried out. It has been determined that the compounds have antimicrobial effect and potential, especially in C. albicans, and that the antimicrobial activity of the encapsulated compounds increases. In chitosan/TPP nanoparticles loaded with compounds; It was determined that the toxicity of L929 fibroblast cells was reduced compared to free phosphazene at 125µM and lower concentrations, and there was no difference in toxicity at higher concentrations. In addition, phosphazene-loaded chitosan particles were observed to increase toxicity to A549 cancer cells compared to free phosphazene.