The effect of KF3B (paenarthrobacter nitroguajacolicus) bacteria used in rooting of oleander cuttings and showing acc deaminase activity at different humidity levels

The study was carried out to examine the effects of humidity levels and KF3B (Paenarthrobacter nitroguajacolicus) bacteria on the plant during rooting of oleander (Nerium oleander L.) cuttings. This study was carried out in the laboratory of the Horticulture Department of Agricultural Faculty of Siirt University. The study have been set up according to the randomized parcel trial design with 3 replications. 30 pieces of cutting were used in each repetition. In addition to inoculation with KF3B bacterial isolate, three different humidity levels (100%, 50%, and 25%) were applied for rooting oleander cuttings. In the study, the effects of bacterial application on basic growth parameters such as rooting percentage, number of root, the longest root length, root width, dry weight of root, fresh weight of root, number of shoots, fresh weight of shoot and dry weight of shoot were examined. According to the results, it was observed that 100% humidity level had the highest effect on rooting percentage in both control and bacterial treatment groups as 80% and 40%, respectively. The rooting of oleander cuttings inoculated with bacteria was less than the rooting of control group cuttings with different levels of humidity control (P<0,001). The effects of treatments on root and shoot fresh weights and shoot dry weights were statistically significant at P<0,001 level and the lowest values in these parameters were obtained from 50% humidity + bacteria treatment. The study reveals that the combination of the efficiency of KF3B bacteria on cuttings rooting with humidity level is lower than the control group plants. Especially at the lowest humidity level of 25%, no rooting and shoot development was observed in oleander cuttings inoculated with KF3B isolate.