Investigation of the effect of hydrogel containing angiogenesis triggering particle on bone healing after tooth extraction in zoledronic acid treated rats.

Zoledronic acid is one of the drugs that cause the development of osteonecrosis of the jaw due to its negative effects on wound healing in soft and hard tissues. The etiology, risk factors, clinical course and effective treatment methods of this condition are still discussed today. The purpose of this study was to understand the effect of PLGA-PEG-PLGA hydrogel containing GS4012 biomaterial, which is a VEGF inducer, on bone healing in rats given zoledronic acid. In our study fourty eight adult male Sprague-Dawley rats were used. Study groups and control groups were formed by randomly distributing the experimental animals into 4 groups, with 12 rats in each group. Each group was divided into two subgroups according to the time of sacrifice. In the first group, bone defects were created only after tooth extraction. In the second group, bone defects were created together with tooth extraction after zoledronic acid injection in rats. In the third group, GS4012 compound in PLGA-PEG-PLGA hydrogel at a dose of 10 µg/kg was applied locally to the bone defects formed with tooth extraction in rats. In the fourth group, after zoledronic acid injection, GS4012 compound in the same dose of PLGA-PEG-PLGA hydrogel was applied locally after tooth extraction. Zoledronic acid was given intraperitoneally to each animal at a dose of 7.5 µg / kg and once a week for a total of 4 times in the groups. In order to evaluate bone healing histopathologically and radiologically, sacrifices were performed at the 4th and 6th weeks after tooth extraction. IBM SPSS Statistics 22 (SPSS IBM, Turkey) programs were used for statistical analysis. As a result, it has shown that GS4012 has the potential to eliminate the negative effects of zoledronic acid on soft tissue by locally sustained release in the PLGA-PEG-PLGA hydrogel.