The investigation of the apoptotic and antiangiogenic effects of noscapi̇ne in human lung cancer (A549) cells

Lung cancer, one of the most important health problems today, is the most common type of cancer that causes death in both women and men. Although A549 lung cells originating from human alveolar carcinoma cells match the type II alveolar cell phenotype, it also has many characteristics that human primary alveolar epithelial cells have. Noscapine consists of the components in the poppy somniferum (opium). It was first isolated from Papaver somniferum (opium) in 1817. It consists of the most abundant opioids found in the opium plant (up to 10% of the total composition) after morphine. It is also known as Narcotine, Nectodon, Nospen, Anarcotine and (archaic) Opiane, and S is an (-) isomer with R stereochemistry (S stereochemistry in phthalid-carbon and R in isoquinoline-carbon). Noskapin is structurally and chemically different from other opium alkaloids such as morphine, codeine, thebaine, papaverine and narcein. Based on this information, the aim of the study was to determine the LD50 (letal dose) value of the pure (commercially obtained) form of noscapine at different concentrations in A549 lung cancer cell lines in vitro by CCK8 cell viability assay and cell proliferation, noscapine in A549 lung cancer cell lines. -To investigate the tumoral activity mechanism with VEGF (Vascular Endothelial Growth Factor) and PARP (Poly (ADP-Ribose) Polymerase) Analyzes. In the study carried out, in the proliferation experiment, the vitality values were determined in direct proportion to the doses in concentrations of 10 ppm, 15 ppm, 20 ppm,25 pm, 40 ppm, 50 ppm, 60 ppm, 65 ppm, 70 ppm, 75 ppm, 80 ppm, 90 ppm and 100 ppm. was found to have decreased and the LD50 value was set at 40 ppm. As a result; Various concentrations of noscapine have been shown to reduce cell proliferation. It is thought that all concentrations of noscapine (20 ppm, 40 ppm and 80 ppm) will reduce angiogenesis and prevent metastasis by lowering VEGF levels. It was observed that the PARP level was lower than the control group. The decrease seen in PARP values suggests that Noscapine leads the cell to apoptotic death.