A troponin biosensor application for determining acute myocard damage

Coronary heart disease is the leading cause of morbidity and mortality among adults in Turkey. It is crucial to diagnose myocardial infarction especially after the coronary bypass surgery. American Heart Association and European Society of Cardiology emphasize that troponins can be used as biochemical markers to diagnose myocardial infarction. The goal of this research is to develop a highly sensitive and rapid biosensor system that can measure troponin T levels in a rapid, sensitive and easy way. In this study, highly sensitive QCM crystals were chemically modified to measure changes in adsorbed mass on the surface and were used to detect the amount of the troponin concentrations. The amount of the troponin proteins was quantified by measuring the change in frequency of the QCM. QCM crystals with immobilized anti-cTnT were tested using different concentrations of troponin molecules. Frequency values of the sensor system were changed proportional to the amount of troponin concentration in the sample, indicating that troponin molecules attach to the surface of the QCM. These results were supported by SEM images. This method presents a rapid and inexpensive biosensor system that monitors troponin levels.