Determinatiion of some phygiological, morphological and chemical changes of salt stress in alternative greens (mibuna, mizuna and komatsuna)

In this study, Mibuna (Brassica rapa var. nipposinica), Mizuna (Brassica rapa var. japonica) and Komatsuna (Brassica rapa L. var. perviridis) were used as plant material. Our experiment was established under controlled conditions in a adjustable climate room with a temperature between + 40 °C and 20 °C. All experiments were performed, 20 °C temperature, 65-70% humidity, 10/14 (light / night) hour photoperiod, 400 µmol m-2s-1 in a climate room. Hoagland hydroponic solution was used by drip irrigation in hydroponic system during emergence and seeding periods then salt stress applications were made. The experiment consists of 3 replications with randomized plots with 3 replicates (Mibuna, Mizuna and Komatsuna) and 4 salt concentrations (control, 50, 100 and 200 mM NaCl). There were 36 parcels in the experiment, 10 plants in each parcel and 360 plants in the whole experiment. During the harvest, leaf damage index, number of leaves, weight of leaf (g), leaf thickness (mm), leaf area (cm2), plant height (cm), root depth (cm), leaf fresh and dry weight (g), relative leaf water content (%), leaf water potential (MPa), membrane damage in leaf cells (%), leaf temperature (°C), chlorophyll content (SPAD value), leaf stoma permeability (mmol m-2s-1), leaf color values (L*, a and b) and macro and micro nutrients content were determined. According to the obtained results from the trial, when the salt rate in the Hoagland hydroponic solution was increased, the number of leaves, leaf weight, leaf area, plant height, root depth, leaf fresh and dry weight, relative leaf water, leaf water potential, chlorophyll amount and macro-micro nutrient elements in the leaves decreased. Therefore, it was determined that all of these criteria decreased with the increasing amount of salt. Contrary to these results, the leaf thickness, membrane damage in leaf cells, leaf temperature, leaf stoma permeability and leaf color values increased with the increasing raios of NaCl.