In vitro micropropagation of Bacopa australis

In this thesis, the roles of culture media including different plant growth regulators (PGR), substrates and culture vessels on mass propagation under in vitro conditions were evaluated and cost effective and rapid in vitro plant regeneration protocol was achieved for the Bacopa australis (L.) plant. In this study; nodal segments of B. australis were used as the explant source for micropropagation. Firstly; the explants were cultured in the semisolid MS medium containing three PGR (BAP, IBA and NAA) combinations. According to the results; the highest number of shoots (17.71 per explant) and the maximum length of shoots (5.53 cm) were observed in the semisolid MS medium containing 3 mg/l BAP and 1 mg/l IBA. Secondly; the responses of the explants to four different substrates were investigated. As a result; the number and length of shoots were significantly higher in the agar solidified culture media than media contain the other substrates. Thirdly; the responses of explants to culture systems were examined. In the light of results; double-phase culture system has come to the fore because of reduction of half of time from the formation of multiple shoots to the obtainment of the whole plant and because it is more economical. The highest number of shoots (13.63 per explant) and roots (18.52 per explant) were determined in the double-phase culture. In fourth phase, the effects of culture vessel types on multiple shoot and root regenerations in nodal explants were examined. When compare different vessels, maximum number of shoots, roots and the highest lenght of roots were recorded in Vitrovent vessels. Finally; in vitro plantlets of B. australis were removed from the culture vessels and transfered into aquarium conditions containing river sand. After 30 days of acclimatization, the survival rate of plantlets were recorded as 100%.