Master'sOpen Access

Morphological and moleculer characterization of some avocado cultivars

2008
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Advisor: Doç. Dr. Yıldız Aka Kaçar

Abstract (EN)

The analyses of RAPD (Randomly Amplified Polymorphic DNA) and SRAP (Sequence Relatad Amplified Polimorphism) marker were carried out on 13 cultivars representing the two ecological races of avocado (Persea americana Mill.) and 7 cultivars of Mexican and Guatemalan hybrids. Eigtheen RAPD primers produced 116 DNA fragments in RAPD assay at the avocado cultivars. Twenty one SRAP primer combination produced 122 bands. A jacard?s similarity coefficient matrix was generated and a dendogram was constructed using UPGMA (Unweighted Pair-Group Metod of Aritmetic Averages) cluster analysis for RAPD and SRAP analysis. Average similarity between genotypes ranged from %46 to %86 with RAPD marker and ranged from %49 to %90 with SRAP marker. The results obtained indicate that the SRAP and RAPD markes used are highly informative and are discussed in term of their implications for avocado germplasm characterization. Tree and leaf characteristics such as tree vigor, tree length, canopy of tree, tree shape, leaf length, color of shoots, leaf shape etc. are evaluated by using IPGRI standarts for twenty avocado cultivars. All quantitative morphological characters analysed by using SAS program.

Author

Aylin Tamam

How to Cite

Aylin Tamam (Master Thesis). Morphological and moleculer characterization of some avocado cultivars, 2008, Çukurova University, Biyoteknoloji Bölümü.

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