Determination of some cardiovascular diseases with biosensor systems and investigation of the potential of them in real samples

Cardiovascular diseases are one of the causes of death in the world. The biosensor systems developed for early detection of these diseases occured due to many different effects and acts a critical role. In this phD thesis, the biosensor systems with disposable electrodes and several electrochemical techniques have been designed to determine CK, ST2 and TnT which are the important biomarkers for early detection of cardiovascular diseases. Electrochemical impedance spectroscopy, cyclic voltammetry, square wave voltammetry and single frequency technique were utilized for immobilization, optimization and characterization studies when biosensors were designed. SEM was used to monitor the morphological changes of the surfaces during the immobilization step. The clinical potential of the biosensors designed by analyzing real serum samples with standard addition method were investigated. It has been observed that the biosensor systems developed for detection of cardiovascular diseases have very high sensitivity, excellent reproducibility and reusability capacity, long shelf life and high sensitivity in the analysis of serum samples.