The relationship of acute and chronic rejection and hla-g geni 3 'utr 14 base polymorphism and SHLA-g level in renal transplant patients

Objective: The most important problem of kidney transplantation is rejection. In this study, determination of sHLA-G level in patients with AUHOTM diagnosed with acute or chronic rejection, determination of HLA-G 3'UTR 14bp polymorphism in the recipient and donor, sHLA-G and polymorphism is aimed to evaluate the relationship between rejection. Method: HLA-G 3'UTR 14b polymorphism was detected using genomic DNA isolation, PCR, agarose jel electrophoresis, DNA sequencing method from peripheral blood samples of kidney transplant patients and donors. 'SPSS for Windows 18' program and Epi Info package version 7 Statcalc were used for data analysis. Student's t-test was used for continuous variables and chi-square was used for categorical variables in comparison of case and control groups. Results: In the case group included in the study, those diagnosed with acute rejection were 41.6%, 76.9% of them were acute antibody-mediated rejection and 23.1% were T-cell-mediated rejection. While those diagnosed with chronic rejection are 58.4%, 94.5% of them are chronic active humoral rejection and 5.5% of them are chronic T cell mediated rejection. In regression analysis, it was determined that the probability of acute rejection in kidney transplant patients increased 1.06 times in a U / ml decrease in sHLA-G level after the transplant compared to the patient before the transplant and 5.44 times in the absence of kinship between the patient and donor. In chronic rejection, the probability of rejection in kidney transplant patients was increased by 1.14 times in a U / ml decrease in sHLA-G level after transplant compared to the patient's pre-transplant. Conclusion: Determination of HLA-G 3'UTR 14bç polymorphism and related sHLA-G level before the transplantation provides for the possibility of rejection. Furthermore, monitoring this polymorphism and sHLA-G level will help identify the patient population with a higher risk of rejection. Key words: HLA-G 3 'UTR 14 bp polymorphism, sHLA-G level, kidney transplant, acute and chronic rejection