Microbiological assay of benzyl penicillin and streptomycin sulphate antibiotics

The antibiotics are effective in healing the bacteriological deseases appeared in human and animals. Thus, the assay studies with improved and identified methods in pharmacopy are performed at laboratory stage before releasing. If the analysis results obtained by studies meet the spesifications, the product can be released as a raw material or finished product. This study covers the microbiological assay studies for the Clemipen-Strep including two active pharmaceutical ingredients called as Streptomycin sulphate and Clemizole Benzyl Penicilin. The Clemipen-Strep is in commerce as a finished product. Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 12228 bacteria were used for Streptomycin sulphate and Benzyl penicilin in this study, respectively. The inhibition zones obtained as a result of 24 hours incubation were measured by zone reader equipment. The assay result were obtained by inserting zone diameter data into potens formulas. Thanks to this method, Streptomycin sulphate and Benzyl penicilin assays in finished product were obtained. The assay results were obtained as 362.7 IU/mg, 362.1 IU/mg, 363.6 IU/mg for Benzylpenicillin and 451.5 IU/mg, 454.9 IU/mg, 453.2 IU/mg for Streptomycin sulphate. The results are appropriate with trend values as 359 IU/mg (% 90?115) for Benzylpenicillin and 449 IU/mg (% 90?115) for Streptomycine sulphate. Keywords: Streptomycin sulphate, Clemizole Benzylpenicillin, Antibiotics, Cylinder Petri Method