Demonstration of fat body cells in culex pipiens using different histochemical techniques

Fat body is a mesodermal originated tissue which possess biosynthetic and metabolic activity and composed of thin layer of one or two cell thickness in insects. Fat body is an endocrine organ for the insects and known that it is equivalent to the liver in vertebrates. In this research, its structure is determined the first time in larvae, pupae and adults of mosquito Culex pipiens, causing many contagious diseases in humans. The structure, cell types and their contents of fat body are shown by using different fixative solutions and various specific histochemical staining methods. The cells of the fat body are one-two lines in some regions, while the others in groups. In the differently stained sections, the cells in the fat body tissue are shown in detail. The most common of these cells are trophocytes and oenocytes which associated with the fat body. Knowing the distribution of the fat body cells and the contents at which stages and in which regions of the animal will be of considerable importance for understanding its physiological process.