Investigation of the effect of sugammadexine on trpm2, trpm4 channels in rat kidney subjected to experimental ischemia-reperfusion

Ischemia-reperfusion (I/R) injury is recognized as an important cause of acute kidney injury. The basic pathophysiology of I/R injury is the oxidative process that occurs after reperfusion of ischemic tissues due to impaired blood flow. This process is an important mechanism leading to impaired cell function, cell death, tissue damage and impaired organ function. The fact that this condition is common and has a high mortality rate is clinically important. In our study, the expression of TRPM2 and TRPM4 channels activated by oxidative stress and its effects on oxidant and antioxidant systems were examined by the use of Sugammadex in rats with renal I/R injury model. In addition, the effects on the apoptotic index calculated using the TUNEL method were also evaluated. In the study, 35 8-10 weeks old male Wistar Albino rats were used. The subjects were divided into 5 groups with 7 animals in each group. The control group (Group I) did not receive any treatment during the experiment. In the sham group (Group 2), the abdomen was opened under general anesthesia, the right kidney was removed and the left renal pedicle was exposed; however, renal clamp was not applied. In the group receiving sugammadex, sugammadex (96 mg/kg) was administered iv at the onset of general anesthesia with the same surgical procedure as in Group 2. In the I/R group (Group IV), the right kidney was removed, the left kidney pedicle was exposed, renal clamp was applied and ischemia was performed for 45 minutes followed by reperfusion for 60 minutes. I/R+Sugammadex group (Group V) received sugammadex at the beginning of reperfusion. At the end of the experiment, the rats were decapitated by taking serum samples and kidney tissues under anesthesia. TAS and TOS values were determined from serum tissue obtained from rats. Immunohistochemical TRPM2, TRPM4 immunoreactivity and TUNEL method were studied from the kidney tissue. In the I/R group, a statistically significant increase was observed in TRPM2, TRPM4 immunoreactivity, serum TOS level and TUNEL positivity, while a decrease was observed in serum TAS value compared to the control group. Compared to the IR group, a statistically significant decrease in TRPM2, TRPM4 immunoreactivity, serum TOS level and TUNEL positivity was observed in the I/R+Sugammadex group, while serum TAS increased.