Detection of biofilm formation incarbapenamase resistance Klebsiella spp.

INTRODUCTION AND AIM: Carbapenem resistant Klebsiella pneumoniae (CR-Kp) become major public health concern due to harboring resistance genes and virulence factors like biofilms. The aim of this study was to detect biofilm formation among (CR-Kp) strains and to determine correlation between carbapenem resistance genes and biofilm formation in K. pneumoniae. MATERIALS AND METHOD: 58 (CR-Kp) involved in this study, identification of the isolates and antibiotic susceptibility testing was performed by using Maldi-TOF MS and VITEK 2 ® system and resistant gene detection held by Gene X-pert system. Microplate method used to detect biofilm formation. The ratio of biofilm formation related to the source of infection and harboring resistance genes were investigated statistically. RESULTS: 98.2% of (CR-Kp)s were positive biofilm producers. 46.5% isolates were moderately biofilm-producer, 37.9% fully established biofilms, 13.7% weak biofilms, 1.72% were non-biofilm producers. Biofilm formation according to infection site: 60.30% surveillance rectal swabs, 27.50% blood, 1.72% sputum, 5.17% wound, 3.44% Urine, 1.72% catheter. KPCs harboring resistance Gene biofilm formation ratio: 42.2% NDM-1, 28.80% OXA 48, 13.30% KPC, 15.5% NDM-1 +OXA 48. DISCUSSION AND CONCLUSION: Almost all CR-Kp isolates are biofilm producers. Surveillance rectal swabs and blood infection source strains, resistance genes NDM-1, OXA 48 KPCs had higher ability forming moderate to fully established biofilms These results indicates that ICU patients and patients who have blood stream infections are at higher risk with high mortality rate. KEY WORDS: Klebsiella pneumonia, Biofilm formation, Resistance Genes, Antibiotic Resistance.