Iron (Fe) deficiency tolerance in tomato recombinant inbreed lines obtained from resistant and sensitive genotypes

The first aim of this study was the determination of iron (Fe) deficiency tolerance levels of the reciprocal recombinant inbred lines (RILs) in tomato. The RILs (F6 generation) have been created using tolerant and sensitive inbred parents for long time studies since 1994. The second aim was the obtaining data which can be used to map the quantative trait loci (QTL) for iron (Fe) deficiency tolerance in tomato. In this study, totally 224 genotypes have been used. 107 genotypes were contained the cytoplasma of the resistant parent-Roza and 117 genotypes were contained the cytoplasma of the sensitive parent-227/1. The plants were grown in growth chamber in hydroponic medium. Thirty-five days old plants were subjected to Fe deficiency stress by 10 mM NaHCO3. The experiment repeated 5 times and each experiment was continued for 57 days. At the end of the experiments, leaf chlorosis, active and total Fe concentrations have been determined. The measured parameters of the RILs have been shown the normal distributions. There were significant differences among the RILs for active, total irons (P? 0.05) and chlorophyll (P?0.001) concentrations. It has been concluded that Fe deficiency tolerance in tomato is inherited quantatively and controlled by multi genes. The findings of this study showed that there were not strong definitive data supporting to the possible cytoplasmic interactions in the inheritance of this trait.