The effect of dietary fat intake during metarnal and maturation periods on the hepatic oxidative strees status in offspring sprague dawley rats

Maternal diet is thought to associated with the determination of obesity, type II diabetes and nonalcoholic fatty liver disease (NAFLD) through oxidative stress and inflammation in the juvenile population. Reactive oxygen species (ROS) contribute to the illness state by harming DNA, lipids, and proteins, driving to a disturbance in cellular homeostasis and an aggregation of harmed molecules. The liver is the controller of vitality body homeostasis and oxidative stress and the liver problems can give rise to numerous metabolic diseases. In this consider, we aimed to determine the impact of different fat-containing diets exposure during maternal and maturation periods on the hepatic oxidative stress in offspring Sprague Dawley rats. In the first part of the study, mother Sprague-Dawley rats were administered with high-fat diet (HFD), standard fat diet (SFD) and low-fat diet (LFD) during the gestational (21 days) and lactation (21 days) periods. The male pups from three main groups were then administered with HFD, SFD and LFD during the maturation period for 120 days in the second part of the study. A total of 54 male rats in nine different groups were utilized in this study. After the sacrification of experimental groups, a part of liver tissues stored at - 80°C in order to determine oxidative stress parameters. In this process, the liver was homogenized and total antioxidant status and total oxidant status in the lysate were determined with commercial kits. Our study suggests that dietary fat intake during maternal and maturation periods can affect the hepatic oxidative stress status in offspring Sprague Dawley rats.