The investigation of CCl1 and P2x7 genes polymorphism pulmoner and extrapulmoner tuberculosis patients in Elaziğ region

Tuberculosis, a leading cause of death worldwide, is characterized by different clinical forms including latent, localized pulmonary infection and extrapulmonary tuberculosis. %90 of people infected with Mycobacterium tuberculosis have latent infection with no symptoms and an immune response that contains the bacilli.Several evidence suggest that host genetics influences susceptibility to tuberculosis. Candidate gene association studies have implicated common polymophisms in genes that may influence the development of tuberculosis. In this study, we aimed to elucidate the role of CCL1 gene in rs159294 polymorphism and P2X7 gene in A1513C polymorphism the etiopathogenesis of tuberculosis.In our study 2 cc peripheral blood samples from 160 tuberculosis disease and 160 healty controls who consulted to department of bosoms disease where Fırat university medicine school in Elazığ region. Bosoms disease department were collected into EDTA anticoagulated tubes and DNA was extracted. rs159294 T/A polymorphism in CCL1 gene and A1513C polymorphism in P2X7 gene was analyzed with PCR- RFLP method.The rates of TT, TA and AA genotypes CCL1 rs159294 T/A polymorphism in 160 tuberculosis disease were calculated as 98 in 160 (%61.25) TT genotypes, 58 in 160 (%36.25) TA genotypes, 4 in 160 (%2.5) AA genotypes, The rates of TT, TA and AA genotypes 71 pulmonary tuberculosis disease were calculated as 50 in 71 (%70.42) TT genotypes, 20 in 71 (%28.16) TA genotypes, 1 in 71 (%1.40) AA genotypes, The rates of TT, TA and AA genotypes 89 extrapulmonary tuberculosis disease were calculated as 48 in 89 (%53.93) TT genotypes, 38 in 89 (%42.69) TA genotypes, 3 in 89 (%3.37) AA genotypes, The rates of TT, TA and AA genotypes 160 control groups were calculated as 100 in 160 (%62.50) TT genotypes, 58 in 160 (%36.25) TA genotypes, 2 in 160 (%1.25) AA genotypes. There were no statistically significant differences between the patient and control groups with regard to genotypes and allel frequencies.The rates of AA, AC and CC genotypes P2X7 A1513C polymorphism in 160 tuberculosis disease were calculated as 91 in 160 (%56.87) AA genotypes, 52 in 160 (%32.50) AC genotypes, 17 in 160 (%10.62) CC genotypes, The rates of AA, AC and CC genotypes 71 pulmonary tuberculosis disease were calculated as 44 in 71 (%61.97) AA genotypes, 18 in 71 (%25.35) AC genotypes, 9 in 71 (%12.67) CC genotypes, The rates of AA, AC and CC genotypes, 89 extrapulmonary tuberculosis disease were calculated as 47 in 89 (%52.80) AA genotypes, 34 in 89 (%38.20) AC genotypes, 8 in 89 (%8.98) CC genotypes, The rates of AA, AC and CC genotypes 160 control groups were calculated as 76 in 160 (%47.50) AA genotypes, 63 in 160 (%39.37) AC genotypes, 21 in 160 (%13.12) CC genotypes. There were no statistically significant differences between the patient and control groups with regard to genotypes and allel frequencies.In conclusion, according to our data the rs159294 polymorphism of CCL1 gene and A1513C polymorphism of P2X7 gene don?t constitute any susceptibility for tuberculosis disease in Elazığ region population.Key words: Tuberculosis, CCL1, P2X7, Polymorphism