Assessment of analgesic activity of Ferula pseudalliacea Rech. F methanol extract in the experimental pain model created in mice

In this study, it was evaluated whether the methanol extract of Ferula pseudalliacea Rech. F. (FP) plant has analgesic activity in mice. The animal material of this study consisted of 60 two-month-old female Swiss albino mice (weighing 25-30 grams). The peripheral analgesic potential of FP was evaluated with writhing test in acetic acid-induced mice and the central analgesic potential was evaluated with the hot plate test. 5 groups (n=6) were created for the writhing test in the study. The groups were as follows; Control Group: 2% Tween 80 (10mL/kg/oral) and Acetic acid (% 0.6, ip), Group 1: Diclofenac sodium (50 mg/kg, orally) + Acetic acid (% 0.6, ip), Group 2: FP (100 mg/kg, orally) + Acetic acid (% 0.6, ip), Group 3: FP (200 mg/kg, orally) + Acetic acid (% 0.6, ip), Group 4: FP (400 mg/kg, orally) + Acetic acid (% 0.6, ip). For the hot plate test, 5 groups were formed with 6 mice in each group. The groups are as follows; Control Group: 2% Tween 80 (10mL/kg) was administered orally, Group 1: (n=6) Diclofenac sodium (50 mg/kg, orally), Group 2: (n=6) FP (100 mg/kg, orally), Group 3: (n=6) FP (200 mg/kg, orally), Group 4: (n=6) FP (400 mg/kg, orally). The results of the writhing test induced with acetic acid show that compared to the control group, diclofenac sodium (p<0.001) and Ferula pseudalliacea extracts show dose-dependent therapeutic significance at 100 mg/kg (p<0.01), 200 and 400 mg/kg (p<0.001) levels. Compared to diclofenac sodium, 100 mg/kg dose of FP showed a significant increase in terms of writhing number (p<0.01); 200 mg/kg and 400 mg/kg doses produced significantly (p<0.05) lower writhing number and showed positive results in terms of therapeutic terms. When the hot plate test results were evaluated, no significance was found in the comparisons between groups (p>0.05). The results of this study; While FP relieved peripheral pain especially at the doses of 200 and 400 mg/kg; It is not effective in terms of central antinociceptive activity. The molecular basis of Ferula pseudalliacea Rech. F. needs to be explained as to which of the bioactive molecules it contains may be responsible for its peripheral antinociceptive activity and whether it can be an alternative product in pain control.