Identification of DNA markers through association mapping of accumulation of P, Cu, K, Mo and Se elements in common bean ( Phaseolus vulgaris L. )

Common bean is an important nutritional source in human diet due to the micronutrients it contains. In this study, 20766 SNP markers were obtained using Genotyping by Sequencing (GBS) method, from 173 common bean genotypes. Analysis that was done by Structure softwere showed that the population is split into two (K=2) main groups. Results from micronutrients analysis performed on common bean genotypes were analyzed with Mixed Linear Model (MLM) on TASSEL (Trait Analysis by Association, Evoluation and Linkage) software, along with SNP marker. According to results of these analysis, 3 markers identified that strongly associated with copper. This study is the first association mapping study about accumulation of Cu, P, K, Mo and Se micronutrients in common bean that uses four different environmental factors (two different locations as Bornova and Menemen throughout two years (2015 and 2016)), and it will help future breeding and marker assisted selection (MAS) studies.