Determination of biochemical activites of Fe3O4 nanoparticle in Saccharomyces cerevisiae

Fe3O4 nanoparticle is one of the metal oxide nanoparticles widely used in many fields with its superior properties. Although studies have been conducted on the toxic effects of these nanoparticles in biological systems, few studies have been conducted to investigate their effects on their biochemical parameters. For this purpose, in the presented study; First of all, for the growth and proliferation of Saccharomyces cerevisiae cell isolated from baker's yeast to be used in the experiment, the stock culture was inoculated into YEDP medium. Two separate experimental groups were formed for each nanoparticle, containing 20 mg/l and 50 mg/l concentrations of Fe3O4 and CuO nanoparticles into the medium. MDA (malondialdehyde), GSH (glutathione), GSSG (oxidized glutathione), total protein, vitamin and phytosterol amounts by HPLC device; fatty acid content was measured by gas chromatography. As a result, lipid peroxidation in yeast cell (d: p<0.001, cd: p<0.0001), GSH (b: p<0.05, cd: p<0.0001) and GSSG (c: p<0.01, d: p<0.001) significantly reduced levels; A remarkable increase was detected in total protein (d: p<0.001, cd: p<0.0001), vitamins, phytosterols and most of the fatty acids. It has been observed that Fe3O4 nanoparticle has different effects on the biochemical and defense system of S. cerevisiae, and it has been observed that Fe3O4 nanoparticles at different doses have effects on reducing stress in the organism. It is thought that the results, especially on the antioxidant defense system, will be a source for similar studies on other living models and will contribute to the knowledge of the literature in studies using in vivo systems.