Identification of cucurbit viruses by das-elisa assays in Gaziantep

Cucurbit growing is effected negatively due to diseases caused by cucurbit viruses.In order to prevent this damage cucurbit viruses were identified by serologically.Due to this study, it is usually difficult to give definitive diagnosis based onsymptoms but occasionaly symptoms; are curling, wrinkling, spot mosaics,yellowing, shape deformation on leaves, smaller leaves than normal, buff -coloredmosaics, observed on younger leaves of cucurbits and stunting , distortion andfruit deformation on the plants. After this, samples collected and taken to laboratoryto determine the virus which caused this symptoms and DAS -ELISA tests wereperformed to determine CMV, CABYV, ZYMV, ToMV, PMMV, PXV, PYV onthe samples collected during July-August in 2004.At the end of this test out of 56 samples, 10 were found to be infected with one ormore virus. As a result of this study 20 samples were infected by CMV and 22samples were infected by ZYMV and 3 samples were infected by PVY.Key words : Cucurbits, Virus Diseases, symptom, Das-Elisai