The evaluation of Goji̇berrys' effect with dietary supplements used in female infertility

Objective: Fertilovit® F35 Plus (FV; Gonodasan AG, Germany) containing B,C,D,E vitamins, coenzyme Q10 with Fe, I and Zn microelements, advised for female preconceptional is a dietary supplement with antioxidant effect. A traditional Chinese medical herb and dietary supplement Gojiberry (GB, Lycium barbarum) having similar content as FV and an antioxidant effect is used as a therapeutic agent/an adjuvant at various illnesses. In this current study, we aimed to determine the effect of GB in single agent and in combination with FV on chronic myeloid leukemia and the pathway of this mechanism action. Materials and methods: Antioxidant capacity determined GB fruits' extracts in single and in combination with FV were applied to K562 leukemia cells for 72 h. Their effects were evaluated by cell viability, apoptotic index (flow cytometry), the levels of apoptotic (Caspases-3,8,9; bax)/ necroapoptotic (RIPK-1) and resistance [Midkine (MK), bcl-2, nf-kappaβ] proteins (ELISA). Anova test was used and p<0.05 was considered statistically significant. Results: Potent inhibition of cell number and viability was determined at the FV group (PFV<0.05). Highest apoptotic index and caspase-8, nf-kappaβ and caspase-3 levels were detected at the FV group (PFV<0.05). Highest bax and bcl-2 levels were determined at the GB group (p<0.05). Highest MK levels were detected at the GB group (PGB>0.05). Conclusion: In this study, it's shown for the first time that FV has anti-cancer effect, the usage of GB with FV shows antagonist effect, actions via extrinsic apoptosis pathway and inhibit a resistance protein MK.