The investigation of the apoptotic and antiangiogenic effects of boron in human lung cancer (A549) cells

Lung cancer, one of the most important health problems today, is the most common cause of death in both men and women. A549 lung cells derived from human alveolar carcinoma cells match the type II alveolar cell phenotype, have many characteristics of human primary alveolar epithelial cells. Boron is used in many fields such as , nuclear, glass, ceramics, pharmaceuticals, detergents, agriculture and its usage areas are increasing day by day. Recent studies have shown that boron is an important element for human health. Based on this information, this study investigated the effect of different concentrations of boric acid in the A549 human lung cancer cell line, by analyzing proliferation assay, TAS (Total Antioxidant Status), TOS (Total Oxidant Status), VEGF (Vascular Endothelial Growth Factor) and PARP (Poly (ADP-). Ribose Polymerase). In the study, the proliferation test showed that the viability values decreased in proportions with doses at 5 mM, 10 mM, 20 mM, 40 mM and 100 mM concentrations and the LD50 value was determined as 20 mM. There was no significant difference between TAS and TOS analysis. In VEGF values, it was observed that VEGF values decreased at 10 mM, 20 mM and 40 mM boric acid concentrations compared to the control group. A significant increase in PARP values was observed at all concentrations of 10 mM, 20 mM and 40 mM. As a result; We may be think 10 mM, 20 mM and 40 mM concentrations of boric acid may decrease angiogenesis by decreasing VEGF levels. As a result of the increase in PARP values, we may be think boric acid leads to cell necrosis at concentrations of 10 mM, 20 mM and 40 mM.