Molecular and physiological study in patients with corona virus in Thi-Qar province Iraq

The current study included 100 patients 50 males and 50 female their age ranged from 10 – 73 years infected with Covid-19 molecularly diagnosed at AL-Imam Hussein Teaching Hospital in Thi-Qar Province for the period from (April to the end of August 2021) and 100 people as a control group . The current study involved some physiological parameters for assessment the physiological effect of Covid-19 compartment with control groups. The results illustrated a significant decrease in the level of hemoglobin, percent of lymphocyte and platelet in patients group, whereas a significant increase in count of total white blood cell in patients group when compare with control group. According to gender of patients the hemoglobin level, count of white blood cell and platelet increased significantly in male group, while percent of lymphocyte increase significantly in female group of patients. According to age groups the results showed a non-significant differences in the hematological parameters. The current results illustrated a significant increase in the level of blood urea, serum creatinine and random blood sugar in patients group. According to gender of patients the blood urea increase significantly in female group, random blood sugar increased significantly in male group, while serum creatinine had not significant difference. According to age groups a significant increase in urea level in patients more than 70 years, but not noted differences in both creatinine and random blood sugar level. The current study recorded the concentration of CRP, ferritin and D. Dimer were significantly higher in patients groups, according to gender the level of CRP and ferritin increased non-significantly compared with male group of patients, while D. Dimer increase significantly in female group. according to age group only CRP increase significantly in first age group compare with other age groups. The current study also included the molecular detection of Covid-19, as the study score 10 viral isolates in the gene bank and knowledge by accession number ( OL372605.1 to OL372614.1), the genetic sequence analysis showed that only one isolate contained a genetic mutation SNPs and has similarity 99.95%.