Biological activity researches of Iris sari Schott ex Baker

Iridaceae is a common family included 92 genus an 6 of them are spreading in our country. According to literatüre survey, there is no research about bioactive ingredients of Iris sari. Therefore, in this study n-hexane, dicloromethane, methanol and water exraction from different parts of I. sari were isolated and their antioxidant, antibacterial and DNA protection activities were studied. To determine antioxidant activity, Rel Assay Diagnostics Kit (TAS, TOS) and DPPH assays; to antibacterial activity; microdilution assay by using 9 different bacterial strains accordingto CSL and; to DNA protection activity, pBR322 plasmid DNA assay were used. According to results, water and methanol extracts showed high antioxidant activity in both method. The highest antioxidant and antibacterial activities were shown by root water extract. The most DNA protection effect was shown by the water and methanol extracts isolated from leaf and flower. In conclusion, it was determined that the endemic plant I. sari contain many active metabolites has antioxidant, antibacterial and DNA protection activities.