Isolation and structural determination of biologically active natural products from three Cephalaria species

This study was aimed to isolation and structural determination of biologically active natural products from Cephalaria hirsuta, Cephalaria procera ve Cephalaria elazigensis var. elazigensis species. Dried and milled plants were extracted with MeOH, n-BuOH:H2O (1:1) and n-hexane. Isolation and purification studies were performed on glycosidic compound-rich n-BuOH phase. Various chromatographic methods (TLC, VLC, MPLC, HPLC, CC) were employed to obtain natural products. After isolation and purification studies; 13 triterpene saponins and 1 iridoid glycoside from C. hirsuta, 8 triterpene saponins from C. procera and 1 iridoid aglycone from C. elazigensis var. elazigensis were obtained. Structures of 23 compounds were determined by 1D- and 2D- NMR techniques. Among the structural designated compounds, 4 compounds in Caprifoliaceae family and 1 compound in Cephalaria genus were identified for the first time. Qualitative and quantitative analysis of glycosidic compounds in studied plants were performed UPLC-ESI-MS/MS instrument for the first time. Validity of applied method was proven by analytical method validation. As a result of analysis on n-BuOH phase, 28 glycosidic compounds in C.hirsuta, 22 glycosidic compounds in C. procera and 4 glycosidic compounds in C. elazigensis var. elazigensis were detected.