Preparation and characterization of cancer cell targeted biomimetic drug carrier systems and research their biopotential

Epithelial ovarian cancer is the most leading of cause of death in women with gynecological cancer. Magnetic agents help to reduce the side effects of pharmaceutical preparations by externally applied magnetic field to tumor tissue. As an antineoplastic agent, doxorubicin is used for treatment of many cancers such as breast, ovarian etc. Erythrocyte vesicles prepared from the body's own cells can eliminate the problem of biocompatibility as a drug delivery system. In this study, doxorubicin loaded and starch and PEGdiacid coated magnetic nanoparticles were synthesized. Magnetic nanoparticles were encapsuled into vesicles that formed by the extruder device from erythrocyte ghost cells that was folate ligand anchored to. Followed by the in vitro drug release studies of nano sized biomimetic drug delivery system, biocompatibility tests and cytotoxicity studies on ovarian cancer cell lines were performed. Based on the data, it was found that biomimetic drug delivery system has a controlled drug release profile, biocompatible and causes cytotoxicity in cells by interacting with folate receptor. In the biodistribution studies carried out on female mice and ex vivo analysis, drug carriers were found to accumulate in the target tissues via folate receptor and magnetic field application. As a result, it is thought that the designed biomimetic doxorubicin transport systems have the potential to provide personalized treatment in ovarian cancer.