Characterization of root knot nematodes resistance Mi-1 gene at some local tomato genotypes

Tomato is vegetable that is produced in large areas all over the world due to it is high economic value and high food usage capacity.It is one of the important factors limiting the production of root knot nematodes in tomato fields.The most effective method of combating pests is to develop them in a durable manner.The Mi-1 gene is a dominant gene in tomato root knot nematodes. In the study, the resistance of the Mi-1 gene, which provides resistance to root knot nematodes in some local tomato genotypes, was investigated.The research was carried out in 2021 in Şırnak University Faculty of Agriculture Horticulture climatic room and Şırnak University Technology and Research Center laboratory. Within the scope of this study, molecular characterization of the Mi-1 gene region was carried out in 20 local tomato genotypes that we obtained from different locations of our country. DNA in 20 local tomato genotypes They were isolated. The Mi-1 gene region was amplified by PCR with the quality genomic DNA obtained. The PCR products obtained by gel electrophoresis technique were visualized. All genotypes were scanned with the Mi23 primer set. As a result of screening with the Mi23 primer set, a single band of 430 bp, homozygous sensitive (mi/mi) was obtained. As a result of the study, a local tomato population collected from different locations of our country was screened for the Mi-1 gene resistance allele.Screening results revealed that the resistance providing allele of the Mi-1 gene in local tomato genotypes is not present in these genotypes.