Manufacturing of novel generation face masks with liposome technology for cosmetotextile applications

In this thesis, cosmetotextiles with functional properties, especially anti-aging and UV protection, were produced. The combined applicability of LbL and liposome methodologies, which allows predicting the further transfer behavior of herbal active ingredients to other substrates such as textiles and skin, was demonstrated. Liposomal vesicles were prepared using the double solvent displacement (DSD) method. Particle size, polydispersity and zeta potential measurements of the liposomes were characterized by the dynamic light scattering (DLS) method. Nonwoven cotton fabrics were coated with liposomal nanoparticles containing milk thistle extracts prepared previously by the LbL method. During the coating process, chitosan acted as a biocompatible and biodegradable polymer to provide the positive charge required for the electrostatic LbL self-assembly. After applying multiple layers of coating, the surface structure and material makeup of the fabrics were examined using scanning electron microscopy with energy dispersive X-ray spectrometry (SEM-EDX). The presence of deposited nanolayers was investigated using Attenuated Total Reflection -Fourier transform infrared spectroscopy (ATR-FTIR). The antioxidant activity of fabrics coated with liposomal milk thistle extracts was measured by CUPRAC (copper ion reducing antioxidant capacity) and DPPH (1,1-diphenyl-2-picryl-hydrazyl) assays. In addition, the inhibitory effect of fabrics coated with milk thistle extracts and liposomal milk thistle extracts on the tyrosinase enzyme was investigated using L-DOPA as a substrate. Moreover, the present thesis study focused on the effect of multilayer fabrics coated with liposomal milk thistle extracts on UPF. In addition, to elucidate the mechanism of anti-tyrosinase action, molecular docking of silibinin and chlorogenic acid, the main components identified in milk thistle extracts, to the target protein 3NM8 was performed. The findings showed that the liposomal population had an average particle size of ~223 nm and a low polydispersity index of 0.398, indicating high particle homogeneity. Additionally, the zeta potentials of liposomes and chitosan were determined to be 28 and +68.23, respectively. EM-EDX results showed that the elements in liposomes changed as more layers were added to the fabric, while ATR-FTIR confirmed that liposomes were successfully added to the nonwoven cotton fabric. The findings indicated significant levels of tyrosinase inhibitory activity and antioxidant ability in fabrics coated with chitosan-enriched camel thorn phenolics. It was also found that the fabric coated with the extracts exhibited very good sun protection ability with a UPF value of 30.55 when compared to untreated and pretreated fabrics.