In vitro investigation of regeneration capability from different of blue spruce (Picea pungens engelm.)

One of the most popular conifers of park, garden and landscape architecture is blue spruce (Picea pungens hoopsi) which is imported plants, is not native plant of Turkey. Import ornamental plants dependence makes come at very high financial cost. In this study, after surface sterilization, in vivo leaf explants of plant were cultured on basal MS medium containing at various combination and concentration of cytokinines and auxins supplemented with 2% sucrose and 0.5% agar as solidifying agent. These cultures were maintained in the climate room at 25 °C±1 temperature with 16 hours fluorescent Although callus induction was the best on 0,1mgl-1 1-Naphthaleneacetic acid + 7.0 mgl-1 6-Benzylaminopurine, somatic embryogenesis were developed on 0,5mgl-1 1-Naphthaleneacetic acid + 3.0 mgl-1 6-Benzylaminopurine and 0,5mgl-1 1-Naphthaleneacetic acid + 7.0 mgl-1 6-Benzylaminopurine approximately in 8 week. However callus and embryos were turned to brownish in two-three weeks, specimens collected from 5-6 years adult trees between May 5-15 was suitable for inciting calli and embryogenesis. Imported blue spruce was very recalcitrant plant for clonal propagation via in vitro tissue culture.Key words: Blue Spruce, Conifers, Clonal, In Vitro, Propagation