Development of biosensor based on surface enhanced raman scattering for multiplex dna analysis

Analysis of DNA is very important in biomedical applications. The sequence of the gene on the DNA can influence the protein structures and thus it can be source of many diseases and genetic problems. Rapid, accurate and sensitive DNA analysis is critically important for the diagnostic of genetic diseases. The most common method is fluorescence based microarrays when the DNA analysis methods are evaluated. However there are some disadvantages of the method. The most important is the overlapping of the fluorescence emission depending on the bandwidth of the spectra. Thus, the complex statistical method should be used for the multiplex analysis that can diminish the performance of multiplexing properties. This is the first time in the literature, the method which is independent from SERS substrate properties and without use of any Raman active dyes for the DNA analysis based SERS on was developed. In this method, the presence of target DNA will be demonstrated by peak shifting of certain peak of the small molecules attached to the SERS substrate surface instead of SERS spectrum obtained in the presence of target DNA. Key Words: DNA analysis, Raman, Biosensor, SERS