Effects of n-ACETYL cysteine in applied experimental malathion on rat kidney and liver tissue transient receptor potential melastatin 2 (TRPM) channels

Organophosphate compounds are commonly used for the control of pests and plants as insecticides. It is known that organophosphates and sub-chronic or chronic toxicity cause histopathologic changes in tissues. Malathion is a wide spectrum organophosphate compound. The objective of the our study was to scrutinize the effects of N-acetyl cysteine on Transient Receptor Potential Melastatin 2 (TRPM2) channel expression in experimental malathion applied rat kidney and liver tissues. 42, 8–10 weeks old, male Wistar albino rats were used in the study. Experimental animals were separated into 7 groups of Control, NAS, Pralidoxime + Atropine, Malathion, Malathion + Pralidoxime + Atropine, Malathion + Pralidoxime + Atropine + NAS and Malathion + NAS, with 6 rats each. N-acetyl cystein was administered as single dose intraperitoneal (i.p.) 100 mg/kg; Pralidoxime 40 mg/kg i.p.; Atropine 2 mg/kg i.p. and single dose orogastric Malathion LD50 (1375 mg/kg)/3 dissolved in corn oil was applied. For determination of apoptotic cells, were used TUNEL method and for immunoreactivity, avidin-biotin-peroxidase method. No differences were observed between the groups in MDA levels, apoptosis, and TRPM2 immunoreactivity in liver tissue in the our study. However, significant increases were observed in MDA levels, apoptosis and TRPM2 immunoreactivity in Malathion and Malathion + NAS groups when compared to the control group in kidney tissue. Also significant reductions were observed in MDA levels, apoptosis and TRPM2 immunoreactivity in Malathion + Pralidoxime + Atropine, Malathion + Pralidoxime + Atropine + NAS groups when compared to Malathion group. As a result, it was concluded that application of experimental malathion did not affect MDA levels, apoptosis, and RPM2 immunoreactivity in rat liver tissue, while it caused significant changes in kidney tissue, TRPM2 channels played an important role in kidney tissue in organophosphate poisoning, and future clinical and extended studies could be conducted on the treatment options about TRPM2. Keywords: Malathion, kidney, liver, N-acetyl Cysteine, TRPM2