Voltammetric determination of cloxacillin and nafcillin with nanowire structured electrodes

It is quite important to develop a rapid and sensitive method for the precise determination of the concentration of the penicillin group antibiotics. In this study, we develop a new method to perform the voltammetric determination of cloxacillin and nafcillin with electrochemically prepared nanowire electrodes. Nanowire electrodes were prepared by electrochemical polymerization and over-oxidation of pyrrole monomer on the electrode surface. As far as our best knowledge, this study is the first study in the literature, which voltammetric determination of chloroxylenol and nafcillin with electrodes. Nanowire pencil graphite electrode, glassy carbon electrode, and platinum electrode were used as working electrodes, Ag/AgCl electrode was used as reference electrode, and Pt wire electrode was used as counter electrode. Two different methods were used in the preparation of nanowire electrodes and the electrodes were prepared by CV method at the potential range of 0.00- (+0.85) V. The surface characterization of the electrodes was performed by scanning electron microscopy (SEM). Optimization studies showed that the optimum pH value was 5, the number of cycles was 8, and the scanning speed was determined as 100 mV/s. Linear responses were obtained with electrodes prepared under optimum conditions. Recovery rates have also been quite good. In conclusion, it was observed that the developed electrodes have many important advantages against the specified drug active ingredients, such as good selectivity, practical applicability in real samples, low detection limit, fast measurement, and being suitable to mass production.